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The process follows a step-by-step protocol:

Any part of a plant (leaf, stem, root) used to initiate culture.

Plant tissue culture, often called in vitro culture, is a fundamental technique in modern botany and biotechnology that involves growing plant cells, tissues, or organs in a sterile, controlled environment on a specialized nutrient medium. This process relies on the biological principle of , which is the unique ability of a single plant cell to regenerate into a whole, functional plant. Core Principles and Laboratory Requirements

Successful tissue culture follows a strict sequential workflow: